IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v42y2006i3id3647-cjgpb.html
   My bibliography  Save this article

Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

Author

Listed:
  • Ulrich Kurt Posselt

    (State Plant Breeding Institute, University of Hohenheim, Stuttgart, Germany)

  • Philippe Barre

    (National Institute of Agronomic Research (INRA), Forage Genetics and Breeding Unit (UGAPF), Lusignan, France)

  • Gintaras Brazauskas

    (Lithuanian Institute of Agriculture, Akademija, Kédainiai, Lithuania)

  • Lesley Beryl Turner

    (Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, United Kingdom)

Abstract

Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).

Suggested Citation

  • Ulrich Kurt Posselt & Philippe Barre & Gintaras Brazauskas & Lesley Beryl Turner, 2006. "Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 42(3), pages 87-94.
    • Handle: RePEc:caa:jnlcjg:v:42:y:2006:i:3:id:3647-cjgpb
    DOI: 10.17221/3647-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3647-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/3647-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/3647-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:42:y:2006:i:3:id:3647-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.