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Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

Author

Listed:
  • R Koprna

    (OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech Republic)

  • V Kučera

    (Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • O Kolovrat

    (OSEVA PRO Ltd., Research Institute of Oilseed Crops, Opava, Czech Republic)

  • M Vyvadilová

    (Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

  • M Klíma

    (Department of Applied Genetics, Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic)

Abstract

Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different "00" quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.

Suggested Citation

  • R Koprna & V Kučera & O Kolovrat & M Vyvadilová & M Klíma, 2005. "Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 41(3), pages 105-111.
    • Handle: RePEc:caa:jnlcjg:v:41:y:2005:i:3:id:3668-cjgpb
    DOI: 10.17221/3668-CJGPB
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    Cited by:

    1. Iva Smýkalová & Martina Větrovcová & Miroslav Klíma & Ivana Macháčková & Miroslav Griga, 2006. "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project "Czech Winter Rape"," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 42(2), pages 58-71.

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