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Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains

Author

Listed:
  • J. Gubiš

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

  • M. Hudcovicová

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

  • L. Klčová

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

  • V. Červená

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

  • K. Bojnanská

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

  • J. Kraic

    (Division of Applied Genetics and Breeding, Research Institute of Plant Production, Piešťany, Slovak Republic)

Abstract

The objective of this paper was to adapt PCR-based detection method for R. secalis and P. teres DNA isolated from pathogens and also from artificially infected juvenile leaves and seeds using pathogen-specific primers. It has been proven that primers specific to P. teres and R. secalis can reliably diagnose pathogen DNA as well as its presence in the mixture with barley DNA. Two primers set for detection of R. secalis were compared. The intensity of the corresponding DNA band after amplification with primer pair RS1-RS3 was higher than that amplified with RS8-RS9. The primer set RS1-RS3 was also used to detect R. secalis in barley seeds. DNA from infected seeds was isolated by two ways - according to the method of Dellaporta et al. (1983) or by the Adgen DNA Extraction System. The DNA extracted using the Adgen kit showed higher quality, however the amplification of the pathogen DNA was accomplished in both cases.

Suggested Citation

  • J. Gubiš & M. Hudcovicová & L. Klčová & V. Červená & K. Bojnanská & J. Kraic, 2004. "Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 40(4), pages 111-117.
    • Handle: RePEc:caa:jnlcjg:v:40:y:2004:i:4:id:3708-cjgpb
    DOI: 10.17221/3708-CJGPB
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    Cited by:

    1. Jozef Gubiš & Martina Hudcovicová & Marcela Gubišová, 2006. "Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 42(3), pages 111-114.

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