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Effect of the disinfectant benzalkonium chloride on Listeria monocytogenes biofilm

Author

Listed:
  • Chao Yang

    (Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China)

  • Xiaochen Zhang

    (Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China)

  • Tao Yu

    (School of Biological Engineering, Xinxiang University, Xinxiang, China)

  • Lin Wang

    (Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China)

  • Zichuan Zhu

    (Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China)

  • Xinjie Fan

    (Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China)

Abstract

Listeria monocytogenes is capable of forming biofilms on the food contact surfaces, increasing the risk of food contamination by this pathogen. The disinfectant benzalkonium chloride (BC) is commonly used to control L. monocytogenes in the food industry. This study aimed to investigate effects of BC on L. monocytogenes biofilms. Biofilm biomass was measured by the microplate method with crystal violet staining. Results from the broth microdilution method showed that the minimum inhibitory concentration (MIC) of BC against L. monocytogenes 10403S was 8 μg.mL-1. Sub-MICs of BC inhibited the biofilm formation and lethal concentrations of BC removed mature biofilms of L. monocytogenes 10403S. The presence of BC reduced extracellular proteins and exopolysaccharides in biofilms. Additionally, upregulation of quorum sensing gene luxS and agrBDCA and downregulation of flagellum motility genes flaA, motA, and motB were observed in the presence of BC. The BC disinfectant has an excellent anti-biofilm activity against L. monocytogenes.

Suggested Citation

  • Chao Yang & Xiaochen Zhang & Tao Yu & Lin Wang & Zichuan Zhu & Xinjie Fan, 2024. "Effect of the disinfectant benzalkonium chloride on Listeria monocytogenes biofilm," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 42(4), pages 295-304.
    • Handle: RePEc:caa:jnlcjf:v:42:y:2024:i:4:id:44-2024-cjfs
    DOI: 10.17221/44/2024-CJFS
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