IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v41y2023i2id234-2022-cjfs.html
   My bibliography  Save this article

Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia

Author

Listed:
  • Zuzana Tomáštíková

    (Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic
    Department of Animal Origin Food and Gastronomic Sciences, University of Veterinary Sciences Brno, Brno, Czech Republic)

  • Lucie Hlucháňová

    (Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic
    Department of Animal Origin Food and Gastronomic Sciences, University of Veterinary Sciences Brno, Brno, Czech Republic)

  • Tereza Gelbíčová

    (Institute of Public Health, Faculty of Medicine, Masaryk University, Brno, Czech Republic)

  • Renáta Karpíšková

    (Institute of Public Health, Faculty of Medicine, Masaryk University, Brno, Czech Republic)

Abstract

Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016-2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.

Suggested Citation

  • Zuzana Tomáštíková & Lucie Hlucháňová & Tereza Gelbíčová & Renáta Karpíšková, 2023. "Listeria monocytogenes clones circulating in the natural environment of the Czech Republic and Slovakia," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 41(2), pages 127-136.
    • Handle: RePEc:caa:jnlcjf:v:41:y:2023:i:2:id:234-2022-cjfs
    DOI: 10.17221/234/2022-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/234/2022-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/234/2022-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/234/2022-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Imran Khan & Jangrez Khan & Sumaira Miskeen & Charles Nkufi Tango & Youn-Seo Park & Deog-Hwan Oh, 2016. "Prevalence and control of Listeria monocytogenes in the food industry - a review," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 34(6), pages 469-487.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:41:y:2023:i:2:id:234-2022-cjfs. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.