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Determining the optimal method for DNA isolation from fruit jams

Author

Listed:
  • Tereza SOVOVÁ
  • Barbora KŘÍŽOVÁ

    (Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic)

  • Jaroslava OVESNÁ

    (Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic)

Abstract

DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods - two kits: DNeasy® Plant Mini Kit and NucleoSpin® Food, and the CTAB method - for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin® Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy® Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Suggested Citation

  • Tereza SOVOVÁ & Barbora KŘÍŽOVÁ & Jaroslava OVESNÁ, 2018. "Determining the optimal method for DNA isolation from fruit jams," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 36(2), pages 126-132.
    • Handle: RePEc:caa:jnlcjf:v:36:y:2018:i:2:id:340-2017-cjfs
    DOI: 10.17221/340/2017-CJFS
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