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Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Author

Listed:
  • Wei Yang

    (College of Life Sciences, China Jiliang University, Hangzhou, P.R. China)

  • Jie Zhu

    (College of Life Sciences, China Jiliang University, Hangzhou, P.R. China)

  • Ming-Qi Liu

    (College of Life Sciences, China Jiliang University, Hangzhou, P.R. China)

  • Xian-Jun Dai

    (College of Life Sciences, China Jiliang University, Hangzhou, P.R. China)

Abstract

To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active group, (E)-2-(2-bromo-phenyl)-2-(methoxyimino) acetic acid methyl ester (OEBr) in kresoxim-methyl and trifloxystrobin. The immunogen and coating antigen were respectively prepared through conjugating the above-mentioned hapten with BSA and OVA by the mixed anhydride and activated ester methods, and polyclonal antibodies were produced by immunised rabbits. An enzyme-linked immunosorbent assay was developed for simultaneous detection of kresoxim-methyl and trifloxystrobin. In ELISA, the antiserum showed high affinity and sensitivity to kresoxim-methyl and trifloxystrobin, and their IC50 value and detection limit (expressed as IC10) were 14.7 and 0.0044 µg/ml, respectively, for kresoxim-methyl, and 22.9 and 0.014 µg/ml, respectively for trifloxystrobin. The cross-reaction rate was below 0.1% for other strobilurin fungicides. Recovery study of ELISA from spiked samples of homogenised peeled oranges (final concentrations of 100, 10, and 1 µg/ml) resulted in recovery levels in the range of 82-104%.

Suggested Citation

  • Wei Yang & Jie Zhu & Ming-Qi Liu & Xian-Jun Dai, 2016. "Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 34(5), pages 429-438.
    • Handle: RePEc:caa:jnlcjf:v:34:y:2016:i:5:id:275-2015-cjfs
    DOI: 10.17221/275/2015-CJFS
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