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Proteolysis in raw milk in relation to microbiological indicators

Author

Listed:
  • Jana Chramostová

    (Dairy Research Institute, Prague, Czech Republic)

  • Oto Hanuš

    (Dairy Research Institute, Prague, Czech Republic)

  • Marcela Klimešová

    (Dairy Research Institute, Prague, Czech Republic)

  • Irena Němečková

    (Dairy Research Institute, Prague, Czech Republic)

  • Petr Roubal

    (Dairy Research Institute, Prague, Czech Republic)

  • Jaroslav Kopecký

    (Dairy Research Institute, Prague, Czech Republic)

  • Radoslava Jedelská

    (Dairy Research Institute, Prague, Czech Republic)

  • Ludmila Nejeschlebová

    (Dairy Research Institute, Prague, Czech Republic)

Abstract

Proteolysis in raw milk is a crucial parameter indicating both cow's mastitis and the technological problems or spoilage risk of final products. However, a suitable analytical method for its early detection in practice is still missing. Thus, we proposed a spectrophotometric determination of milk proteolysis equivalent (MPE). We tested this method on 104 bovine raw milk samples in relation to their somatic cell count (SCC) as an indicator of native proteolysis, and the total count of mesophilic bacteria (TCMB) and the total count of psychrotrophic bacteria (TCPB) as indicators of microbial proteolysis. Correlation coefficients between log TCMB and MPE and log TCPB and MPE were 0.3651 and 0.4152, respectively (both P < 0.001). SCC was not correlated with MPE (P > 0.05). We estimated the MPE limit indicating an incipient risk of proteolysis in the range from 0.9366 to 1.02 mmol/l. The determination of MPE seems to be a promising method applicable in the control of raw milk.

Suggested Citation

  • Jana Chramostová & Oto Hanuš & Marcela Klimešová & Irena Němečková & Petr Roubal & Jaroslav Kopecký & Radoslava Jedelská & Ludmila Nejeschlebová, 2016. "Proteolysis in raw milk in relation to microbiological indicators," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 34(4), pages 306-312.
    • Handle: RePEc:caa:jnlcjf:v:34:y:2016:i:4:id:64-2016-cjfs
    DOI: 10.17221/64/2016-CJFS
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    References listed on IDEAS

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    1. Irena Němečková & Marta Pechačová & Petr Roubal, 2009. "Problems with detection of proteolytic microorganisms and their undesirable activities in milk," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 82-89.
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