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Effect of fungicide treatment on Fusarium culmorum and Tri genes transcription in barley malt

Author

Listed:
  • Jozef Pavel

    (Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Kateřina Vaculová

    (Agrotest Fyto, Ltd., Kroměříž, Czech Republic)

  • Zuzana Faltusová

    (Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Ladislav Kučera

    (Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Irena Sedláčková

    (Agrotest Fyto, Ltd., Kroměříž, Czech Republic)

  • Ludvík Tvarůžek

    (Agrotest Fyto, Ltd., Kroměříž, Czech Republic)

  • Jaroslava Ovesná

    (Division of Crop Genetics and Breeding, Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

Malting barley grains are essential components in the beer production. Fusarium infection can have severe effects on malt and beer, because it may inhibit the enzymatic activity in malt and may induce the occurrence of gushing and changes in the colour and flavour of the finished beer. We examined the growth of the filamentous fungi Fusarium culmorum in artificially infected and non-infected barley malting grains during the first steps of the malting process and under the effects of fungicide pretreatment (Hutton and Prosaro 250 EC) of barley plants. Our study focused on the fungi growth in two distinct barley malting cultivars Bojos and Malz. Fusarium growth was investigated by quantitative real-time PCR using TagMan MGB probes. Furthermore, we focused on the Tri5 and Tri6 genes because they play the most important roles in trichothecene biosynthesis. Surprisingly, the higher transcription activity of the Tri genes was found in the fungicide-treated cultivar Malz as compared with untreated cultivars.

Suggested Citation

  • Jozef Pavel & Kateřina Vaculová & Zuzana Faltusová & Ladislav Kučera & Irena Sedláčková & Ludvík Tvarůžek & Jaroslava Ovesná, 2015. "Effect of fungicide treatment on Fusarium culmorum and Tri genes transcription in barley malt," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 33(4), pages 326-333.
    • Handle: RePEc:caa:jnlcjf:v:33:y:2015:i:4:id:541-2014-cjfs
    DOI: 10.17221/541/2014-CJFS
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