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Direct identification of bifidobacteria from probiotic supplements

Author

Listed:
  • Věra Bunešová

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Eva Vlková

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Vojtěch Rada

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Petra Hovorková

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Šárka Musilová

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Vladimír Kmeť

    (Institute of Animal Physiology of Slovak Academy of Sciences, Košice, Slovak Republic)

Abstract

The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.

Suggested Citation

  • Věra Bunešová & Eva Vlková & Vojtěch Rada & Petra Hovorková & Šárka Musilová & Vladimír Kmeť, 2014. "Direct identification of bifidobacteria from probiotic supplements," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 32(2), pages 132-136.
    • Handle: RePEc:caa:jnlcjf:v:32:y:2014:i:2:id:291-2013-cjfs
    DOI: 10.17221/291/2013-CJFS
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