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In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract

Author

Listed:
  • Yu-Hua Wong

    (Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia)

  • Chin-Ping Tan

    (Department of Food Technology, Faculty of Food Science and Technology, Putra University Malaysia, Serdang, Selangor, Malaysia)

  • Kamariah Long

    (Malaysian Agricultural Research & Development Institute (MARDI), Kuala Lumpur, Malaysia)

  • Kar-Lin Nyam

    (Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia)

Abstract

We investigate the biostability of phenolic acids from a kenaf (Hibiscus cannabinus L.) seed extract using an in vitro model simulating the physicochemical (pH, temperature and bile salts) and biological (gastric and pancreatic enzymes) gastrointestinal conditions. Some of the antioxidants in the kenaf seed extract were not relatively stable in the intestinal phase of the gastrointestinal tracts. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and total phenolic content (TPC) assays displayed similar trends as the biostability of phenolic acids, which decreased during the digestion process. The overall percentage loss of selected phenolic acids was 8.4-49.4% in the intestinal phase. For the overall in vitro digestion system, significant correlations between phenolic acids, total phenolic content (TPC) and antioxidant activity (DPPH) were obtained in all digestion phases with the exception of the gastric phase.

Suggested Citation

  • Yu-Hua Wong & Chin-Ping Tan & Kamariah Long & Kar-Lin Nyam, 2014. "In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 32(2), pages 177-181.
  • Handle: RePEc:caa:jnlcjf:v:32:y:2014:i:2:id:222-2013-cjfs
    DOI: 10.17221/222/2013-CJFS
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    References listed on IDEAS

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    1. Jasna M. Čanadanović-Brunet & Sladjana S. Savatović & Gordana S. Ćetković & Jelena J. Vulić & Sonja M. Djilas & Siniša L. Markov & Dragoljub D. Cvetković, 2011. "Antioxidant and antimicrobial activities of beet root pomace extracts," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(6), pages 575-585.
    2. Safeer AHMED & Faria SHAKEEL, 2012. "Antioxidant activity coefficient, mechanism, and kinetics of different derivatives of flavones and flavanones towards superoxide radical," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(2), pages 153-163.
    3. Zoran Herceg & Edita Juraga & Brankica Sobota-Šalamon & Anet Režek-Jambrak, 2012. "Inactivation of mesophilic bacteria in milk by means of high intensity ultrasound using response surface methodology," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(2), pages 108-117.
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