IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v32y2014i1id432-2012-cjfs.html
   My bibliography  Save this article

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

Author

Listed:
  • Kate Cristina Blanco

    (Department of Biochemistry and Microbiology, Biological Sciences Institute, UNESP - University Estadual Paulista, Rio Claro, Brazil)

  • Flávio Faria De Moraes

    (Chemical Engineering Department, UEM - University Estadual de Maringá, Maringá, Brazil)

  • Natalia Sozza Bernardi

    (Department of Biochemistry and Microbiology, Biological Sciences Institute, UNESP - University Estadual Paulista, Rio Claro, Brazil)

  • Mary Helen Palmuti Braga Vettori

    (Department of Biochemistry and Microbiology, Biological Sciences Institute, UNESP - University Estadual Paulista, Rio Claro, Brazil)

  • Rubens Monti

    (Department of Food and Nutrition, Faculty of Pharmaceutical Sciences, UNESP - University Estadual Paulista, Araraquara, Brazil)

  • Jonas Contiero

    (Department of Biochemistry and Microbiology, Biological Sciences Institute, UNESP - University Estadual Paulista, Rio Claro, Brazil)

Abstract

The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using β-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cyclodextrin glycosyltransferase were 55°C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.

Suggested Citation

  • Kate Cristina Blanco & Flávio Faria De Moraes & Natalia Sozza Bernardi & Mary Helen Palmuti Braga Vettori & Rubens Monti & Jonas Contiero, 2014. "Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 32(1), pages 48-53.
    • Handle: RePEc:caa:jnlcjf:v:32:y:2014:i:1:id:432-2012-cjfs
    DOI: 10.17221/432/2012-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/432/2012-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/432/2012-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/432/2012-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:32:y:2014:i:1:id:432-2012-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.