IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v31y2013i6id494-2011-cjfs.html
   My bibliography  Save this article

Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

Author

Listed:
  • Orlando G. Nagel

    (Department of Basic Science, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina)

  • Maria Pilar Molina

    (Department of Animal Science, Polytechnic University of Valencia, Valencia, Spain)

  • Rafael L. Althaus

    (Department of Basic Science, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina)

Abstract

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (24-1). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

Suggested Citation

  • Orlando G. Nagel & Maria Pilar Molina & Rafael L. Althaus, 2013. "Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 31(6), pages 627-632.
    • Handle: RePEc:caa:jnlcjf:v:31:y:2013:i:6:id:494-2011-cjfs
    DOI: 10.17221/494/2011-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/494/2011-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/494/2011-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/494/2011-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:31:y:2013:i:6:id:494-2011-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.