IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v31y2013i4id316-2012-cjfs.html
   My bibliography  Save this article

Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources

Author

Listed:
  • Priti KHEMARIYA

    (Post Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), India)

  • Sudhir SINGH

    (Post Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), India)

  • Gopal NATH

    (Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), India)

  • Anil K. GULATI

    (Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), India)

Abstract

Eight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy sources. All eight strains of L. lactis subsp. lactis were able to produce zones of inhibition against the Lactobacillus acidophilus NCDC 015. The antimicrobial agent produced by the isolates inhibited the growth of a range of related lactic acid bacteria and certain Gram positive food-borne microorganisms. The antimicrobial agent, i.e. nisin, produced by the strains was confirmed by PCR amplification of nisin gene sequences of 174 bp size. Antibiotic susceptibility test to 21 different types of antibiotics was evaluated. All the isolates were resistant to fosfomycin, cefepime, amikacin, kanamycin, neomycin, nalidixic acid, pipemidic acid, norfloxacin, sulphadiazine, colistin, polymixin, teicoplanin, nystatin, and amphotericin B but susceptible to ampicillin, erythromycin, spiramycin, spectinomycin, ciprofloxacin, rifampicin, and trimethoprim.

Suggested Citation

  • Priti KHEMARIYA & Sudhir SINGH & Gopal NATH & Anil K. GULATI, 2013. "Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 31(4), pages 323-331.
    • Handle: RePEc:caa:jnlcjf:v:31:y:2013:i:4:id:316-2012-cjfs
    DOI: 10.17221/316/2012-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/316/2012-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/316/2012-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/316/2012-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:31:y:2013:i:4:id:316-2012-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.