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Contents of extractable and non-extractable polyphenols in the leaves of blueberry

Author

Listed:
  • Anwei Cheng

    (Institute of Agri-food Science and Technology, Shandong Academy of Agricultural Science, Jinan, P.R. China)

  • Xiangyan Chen

    (Institute of Agri-food Science and Technology, Shandong Academy of Agricultural Science, Jinan, P.R. China)

  • Wenliang Wang

    (Institute of Agri-food Science and Technology, Shandong Academy of Agricultural Science, Jinan, P.R. China)

  • Zhiqing Gong

    (Institute of Agri-food Science and Technology, Shandong Academy of Agricultural Science, Jinan, P.R. China)

  • Lina Liu

    (Institute of Agri-food Science and Technology, Shandong Academy of Agricultural Science, Jinan, P.R. China)

Abstract

The contents of extractable polyphenols (EPP), non-extractable polyphenols (NEPP), extractable proanthocyanidins (EPAC), non-extractable proanthocyanidins (NEPAC) and extractable anthocyanidins (EAC) in blueberry leaves were investigated. An experiment was conducted to analyse the effect of solvent types (methanol and ethanol), concentration (50 and 70%) and pH (2 and 6) on the extraction of bioactive compounds. Total extractable polyphenols (TEPP), total extractable proanthocyanidins (TEPAC), and total extractable anthocyanidins (TEAC) were analysed in methanol/ethanol/water extracts, NEPP were determined in acidic hydrolysates and NEPAC were quantified by depolymerisation in HCl/butanol. The results showed that ethanol and methanol did not affect the extraction of TEPP, while methanol was better for TEPAC and TEAC. The contents of TEPP and TEAC in 50% solvent were higher than those in 70% solvent. When solvent pH dropped to 2, the contents of EPP and EPAC were increased, while the EAC content was decreased.

Suggested Citation

  • Anwei Cheng & Xiangyan Chen & Wenliang Wang & Zhiqing Gong & Lina Liu, 2013. "Contents of extractable and non-extractable polyphenols in the leaves of blueberry," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 31(3), pages 275-282.
  • Handle: RePEc:caa:jnlcjf:v:31:y:2013:i:3:id:272-2012-cjfs
    DOI: 10.17221/272/2012-CJFS
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