IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v31y2013i2id125-2012-cjfs.html
   My bibliography  Save this article

Differentiation of Lactobacillus species by ARDRA

Author

Listed:
  • Kateřina Kšicová

    (Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic, 2Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic, 3Veterinary Research Institute, Brno, Czech Republic)

  • Marta Dušková
  • Renáta Karpíšková

Abstract

The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (AluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.

Suggested Citation

  • Kateřina Kšicová & Marta Dušková & Renáta Karpíšková, 2013. "Differentiation of Lactobacillus species by ARDRA," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 31(2), pages 180-188.
    • Handle: RePEc:caa:jnlcjf:v:31:y:2013:i:2:id:125-2012-cjfs
    DOI: 10.17221/125/2012-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/125/2012-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/125/2012-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/125/2012-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:31:y:2013:i:2:id:125-2012-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.