IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v31y2013i1id107-2012-cjfs.html
   My bibliography  Save this article

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Author

Listed:
  • Eliška VÁCLAVÍKOVÁ

    (Department of Food Preservation, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

  • František KVASNIČKA

    (Department of Food Preservation, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

Abstract

Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many of the marketed products. We present here a capillary isotachophoretic method for the determination of glucosamine and chondroitin sulfate in dietary supplements. Cationic analysis of glucosamine was performed with a leading electrolyte consisting of 10mM NH4OH + 20mM acetic acid. As the leading electrolyte for anionic analysis of chondroitin sulphate, a mixture of 5mM HCl + 10mM glycylglycine + 0.05% of 2-hydroxyethylcellulose was used. The solution of 10mM citric acid served as the terminating electrolyte for both glucosamine and chondroitin sulfate analyses. The analytes were detected by conductivity and UV detectors. The characteristics of the method,, i.e., linearity, accuracy, repeatability, and quantitation limit, were evaluated. On a set of 35 samples of marketed dietary supplements did we prove that the capillary isotachophoresis is a suitable method for the routine analysis of glucosamine and chondroitin sulfate.

Suggested Citation

  • Eliška VÁCLAVÍKOVÁ & František KVASNIČKA, 2013. "Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 31(1), pages 55-65.
    • Handle: RePEc:caa:jnlcjf:v:31:y:2013:i:1:id:107-2012-cjfs
    DOI: 10.17221/107/2012-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/107/2012-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/107/2012-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/107/2012-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:31:y:2013:i:1:id:107-2012-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.