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Optimisation of antioxidant extraction from lemon balm (Melissa officinalis)

Author

Listed:
  • Miroslav Ondrejovič

    (Biocentre Modra, Food Research Institute, Modra, Slovak Republic)

  • Filip Kraic

    (Department of Biotechnology, Faculty of Natural Sciences, University of ss. Cyril and Methodius, Trnava, Slovak Republic)

  • Hana Benkovičová

    (Department of Biotechnology, Faculty of Natural Sciences, University of ss. Cyril and Methodius, Trnava, Slovak Republic)

  • Stanislav Šilhár

    (Biocentre Modra, Food Research Institute, Modra, Slovak Republic)

Abstract

The effects of the propan-2-ol proportion in the extraction solvent (PPES), solid to liquid ratio (SLR), and extraction temperature on the extraction yield of antioxidants measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and β-carotene-linoleic acid bleaching inhibition activity (BCLM) were evaluated. Secondly, total polyphenol and flavonoid contents were determined to find possible relations of these parameters with antioxidant activity. The optimal conditions for the extraction were determined using response surface methodology (RSM). The optimal conditions for the extraction of antioxidants measured by radical scavening activity (DPPH) were PPES 50.2% (v/v), 33.8°C, and SLR 1:147 (w/v). The optimal conditions for the extraction of antioxidants measured by BCLM were PPES 1.15% (v/v), 61.8°C, and SLR 1:153 (w/v). The optimal conditions for the extraction of total polyphenols and total flavonoids were 23.3% (v/v) (PPES), 67.5°C, 1:148 (w/v) (SLR); 1.15% (v/v) (PPES), 80°C, 1:179 (w/v) (SLR); respectively. The experimental values agreed with the predicted ones within a 95% confidence interval.

Suggested Citation

  • Miroslav Ondrejovič & Filip Kraic & Hana Benkovičová & Stanislav Šilhár, 2012. "Optimisation of antioxidant extraction from lemon balm (Melissa officinalis)," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(4), pages 385-393.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:4:id:436-2010-cjfs
    DOI: 10.17221/436/2010-CJFS
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