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Bacteriocin activity of enterococci and presence of genes related to pathogenesis

Author

Listed:
  • Krina Trivedi

    (Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • Radmila Sedmíková

    (Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • Renáta Karpíšková

    (Department of Milk Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
    National Institute of Public Health, Brno, Czech Republic)

Abstract

In total 228 enterococci strains isolated from food were studied. Enterococcus faecalis, E. faecium, and E. casseliflavus were found to be the dominant strains while E. durans and E. mundtii were present in a smaller extent. Antimicrobial activity determined by double layer technique revealed that 150 (65.7%) strains showed antimicrobial activity against the individual tested pathogenic strains of Listeria monocytogenes, Staphylococcus aureus, and methicilin resistant S. aureus (MRSA). Cell-free neutralised supernatants (CFNS) were prepared from 150 potential bacteriocin producers. Of these 150, CFNS 107 (71.3%) strains were active in the bacteriocin production against one or more pathogenic strains tested. S. aureus and MRSA were found to be more sensitive to the antimicrobial substances than L. monocytogenes. Multiplex PCR for the detection of seven virulence genes in bacteriocin producing strains showed that 47.6% of strains were able to amplify one or more virulence genes. E. faecalis was the most virulent species. The presence of tyrdc gene was seen in all bacteriocin producing strains. None of the strains carried genes encoding the resistance to vancomycin.

Suggested Citation

  • Krina Trivedi & Radmila Sedmíková & Renáta Karpíšková, 2012. "Bacteriocin activity of enterococci and presence of genes related to pathogenesis," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(4), pages 330-335.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:4:id:181-2011-cjfs
    DOI: 10.17221/181/2011-CJFS
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    References listed on IDEAS

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    1. K. Trivedi & I. Borkovcová & R. Karpíšková, 2009. "Tyramine Production by Enterococci from Various Foodstuffs: A Threat to the Consumers," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 357-360.
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