IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v30y2012i3id525-2010-cjfs.html
   My bibliography  Save this article

Cow's milk proteins immunoreactivity and allergenicity in processed food

Author

Listed:
  • Barbara Wróblewska

    (Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Anna Kaliszewska

    (Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

Abstract

The immunoreactivity and allergenicity of proteins present in processed food (UHT milk, yoghurt, hard cheese, cottage cheese, biscuit, and sausage intended for children) were determined in this study. Proteins were characterised by SDS-PAGE electrophoresis. The changes in immunoreactivity were compared by enzyme-linked immunosorbent assay (ELISA) using polyclonal rabbit antibodies specific to α-lactalbumin (α-la), β-lactoglobulin (β-lg), α-, β-, and κ-casein. The allergenicity was determined with human pooled sera from CMA allergic patients by ELISA and immunoblot. The results have shown that the allergenicity of the food products is mainly correlated with bovine serum albumin (BSA), lactofferin (LF), and α-casein or the products of non-specific reactions between carbohydrate and proteins (e.g. lactosylation).

Suggested Citation

  • Barbara Wróblewska & Anna Kaliszewska, 2012. "Cow's milk proteins immunoreactivity and allergenicity in processed food," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(3), pages 211-219.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:3:id:525-2010-cjfs
    DOI: 10.17221/525/2010-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/525/2010-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/525/2010-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/525/2010-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jaromír DUCHÁČEK & Luděk STÁDNÍK & Martin PTÁČEK & Jan BERAN & Monika OKROUHLÁ & Jaroslav ČÍTEK & Roman STUPKA, 2014. "Effect of cow energy status on the hypercholesterolaemic fatty acid proportion in raw milk," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 32(3), pages 273-279.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:30:y:2012:i:3:id:525-2010-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.