IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v30y2012i1id7-2011-cjfs.html
   My bibliography  Save this article

Outdoor environment as a source of Listeria monocytogenes in food chain

Author

Listed:
  • Tereza Gelbíčová

    (National Institute of Public Health Prague, Brno, Czech Republic
    Faculty of Science, Masaryk University Brno, Brno, Czech Republic)

  • Renáta Karpíšková

    (National Institute of Public Health Prague, Brno, Czech Republic
    University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

Abstract

We monitored the presence of Listeria monocytogenes in environmental sources and to evaluate the phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 (11.2%) of the 107 samples from the wild, farm environment, and vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 (33.3%) of 12 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes.

Suggested Citation

  • Tereza Gelbíčová & Renáta Karpíšková, 2012. "Outdoor environment as a source of Listeria monocytogenes in food chain," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(1), pages 83-88.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:7-2011-cjfs
    DOI: 10.17221/7/2011-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/7/2011-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/7/2011-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/7/2011-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:7-2011-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.