IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v30y2012i1id226-2010-cjfs.html
   My bibliography  Save this article

Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012

Author

Listed:
  • Marian Urban

    (Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic)

  • Miloš Beran

    (Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic)

  • Lubomír Adámek

    (Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic)

  • Josef Drahorád

    (Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic)

  • Petr Molík

    (Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic)

  • Kristina Matušová

    (AMR Amaranth, Blansko, Czech Republic)

Abstract

Cyclodextrins (CDs) are synthesised by bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives. The production of α-, β-, and γ-CDs by CGTase from Paenibacillus mace-rans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important α-, β-, and γ-CDs were detected chromatographically after the hydrolysis of the maize and amaranth (Amaranthus cruentus) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs production because of the high dispersibility, high starch-granule susceptibility to amylases, and the exceptionally high amylopectin content.

Suggested Citation

  • Marian Urban & Miloš Beran & Lubomír Adámek & Josef Drahorád & Petr Molík & Kristina Matušová, 2012. "Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans CCM 2012," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(1), pages 15-20.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:226-2010-cjfs
    DOI: 10.17221/226/2010-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/226/2010-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/226/2010-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/226/2010-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:226-2010-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.