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Genetic determinants of mycotoxin synthesis in genus Fusarium

Author

Listed:
  • Hana Havránková

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
    Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Jarmila Pazlarová

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

  • Jaroslava Ovesná

    (Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic)

Abstract

The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression - Tri4, Tri10 - involved in the production of Fusarium mycotoxin deoxynivalenol. Reference genes β-tub and UBC were found to be suitable for the expression level normalisation using the program geNorm. The level of expression of the target genes was analysed on four isolates of Fusarium graminearum grown in vitro. In all four isolates, Tri10 gene expression level was lower than that of Tri4 gene expression.

Suggested Citation

  • Hana Havránková & Jarmila Pazlarová & Jaroslava Ovesná, 2011. "Genetic determinants of mycotoxin synthesis in genus Fusarium," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(SpecialIs), pages 86-93.
    • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:specialissue:id:314-2011-cjfs
    DOI: 10.17221/314/2011-CJFS
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