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Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products

Author

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  • Matej Pospiech

    (Department of Vegetable Foodstuffs and Plant Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Bohuslava Tremlová

    (Department of Vegetable Foodstuffs and Plant Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Eva Renčová

    (Veterinary Research Institute, Brno, Czech Republic)

  • Zdeňka Randulová

    (Department of Vegetable Foodstuffs and Plant Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Zuzana Řezáčová Lukášková

    (Department of Vegetable Foodstuffs and Plant Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Jana Pokorná

    (Department of Vegetable Foodstuffs and Plant Production, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

Abstract

This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. Histochemical and immunohistochemical methods were used for microscopical examination. From a group of 252 meat products, each sample was examined for soya proteins by ELISA, histochemical, and immunohistochemical methods. The products came from the following categories: cooked sausages, ham, dry cooked sausages, and fermented sausages. The results showed that the highest accuracy was achieved by immunohistochemical examination. However, in the category of cooked sausages, this result was not statistically significant. Since the results in the individual categories differed, our results demonstrate that one single method does not always provide reliable and completely objective results. Immunohistochemical methods seem to be the most suitable for the verification of the reference immunochemical method results and prevention of false results.

Suggested Citation

  • Matej Pospiech & Bohuslava Tremlová & Eva Renčová & Zdeňka Randulová & Zuzana Řezáčová Lukášková & Jana Pokorná, 2011. "Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(5), pages 471-479.
    • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:5:id:209-2010-cjfs
    DOI: 10.17221/209/2010-CJFS
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    1. Matej POSPIECH & Bohuslava TREMLOVÁ & Eva RENČOVÁ & Zdeňka RANDULOVÁ, 2009. "Immunohistochemical detection of soya protein - optimisation and verification of the method," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(1), pages 11-19.
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    Cited by:

    1. Javad Sadeghinezhad & Bahador Hajimohammadi & Farkhondeh Izadi & Fatemeh Yarmahmoudi & Rocco Latorre, 2015. "Evaluation of the morphologic method for the detection of animal and herbal content in minced meat," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 33(6), pages 564-569.

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