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Application of luminescent ATP rapid checks at ready-to-eat foods producing plant

Author

Listed:
  • Pasquale Caputo

    (Department of Metallurgic Science, Electrochemistry and Chemical Techniques, University of Bologna, Bologna, Italy)

  • Elida N. Ferri

    (Department of Metallurgic Science, Electrochemistry and Chemical Techniques, University of Bologna, Bologna, Italy)

  • Stefano Girotti

    (Department of Metallurgic Science, Electrochemistry and Chemical Techniques, University of Bologna, Bologna, Italy)

  • Simone Gozzi

    (CAMST Scrl, Bologna, Italy)

  • Pasquale Saracino

    (CAMST Scrl, Bologna, Italy)

Abstract

Bioluminescent ATP determination has been applied at a ready-to-eat meals producer plant as a screening tool to evaluate the cleaning procedures and identify possible sources of microbial contamination. Luminescent ATP test revealed the risk situations in few minutes, any time it was supposed useful, and on site. All data were confirmed by the plate count method and immediate interventions were undertaken in risk situations, concerning not perfectly cleaned surfaces and cross-contamination between the areas for different types of foods. Various departments have been checked: cold stores, raw food manipulation areas, kitchens, coolers, and packaging areas. Critical situations were revealed in the fresh foods cold storage areas, which were consequently treated by more intensive cleaning procedures, and the raw food manipulation areas, which were physically separated by walls, were more intensively cleaned with exchanges between the various areas strictly regulated. Intervention effectiveness was confirmed by repeated controls using portable ATP assay, since the evaluation of the relationship between CFU and RLU gave good results, with an r2 value of 0.83.

Suggested Citation

  • Pasquale Caputo & Elida N. Ferri & Stefano Girotti & Simone Gozzi & Pasquale Saracino, 2011. "Application of luminescent ATP rapid checks at ready-to-eat foods producing plant," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(4), pages 382-390.
    • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:4:id:197-2010-cjfs
    DOI: 10.17221/197/2010-CJFS
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