IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v28y2010i5id228-2009-cjfs.html
   My bibliography  Save this article

Kinetics of hydrolysis of egg white protein by pepsin

Author

Listed:
  • Chang-Qing Ruan

    (College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China
    College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, P. R. China)

  • Yu-Jie Chi

    (College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China)

  • Rui-Dong Zhang

    (College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China)

Abstract

Taking into account the enzyme inactivation and substrate inhibition, the bioreaction mechanism and kinetics characteristic of egg white protein (EWP) enzymatic hydrolysis by pepsin were investigated. A logarithmic equation h = (1/b) ln (1 + abt) indicating the relationship between the degree of hydrolysis (DH) and time was established. For EWP-pepsin system, the reaction mechanism could be deduced from a series of experimental results at different temperatures, pH values, substrate concentrations, and enzyme concentrations. The reaction kinetics and thermodynamic constants (KS = 3916.5 g/l, k2 = 17 202.86 min-1, kd = 21 962.03, Ea = 56.89 kJ/mol, Ed = 51.99 kJ/mol) were responsible for the empirical equations. The results of nonlinear regression of the proposed kinetic model agreed with the experimental data, i.e. the average relative error was less than 5%. As a conclusion, the kinetic equations can be used to fit the enzymatic hydrolysis process of egg white protein and to optimise the operating parameters of bioactive peptides preparation for the bioreactor design.

Suggested Citation

  • Chang-Qing Ruan & Yu-Jie Chi & Rui-Dong Zhang, 2010. "Kinetics of hydrolysis of egg white protein by pepsin," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 28(5), pages 355-363.
    • Handle: RePEc:caa:jnlcjf:v:28:y:2010:i:5:id:228-2009-cjfs
    DOI: 10.17221/228/2009-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/228/2009-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/228/2009-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/228/2009-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:28:y:2010:i:5:id:228-2009-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.