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Optimisation of ultrasonic-assisted protein extraction from brewer's spent grain

Author

Listed:
  • De-Song Tang

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

  • Ying-Juan Tian

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

  • Yuan-Zhe He

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

  • Lin Li

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

  • Song-Qing Hu

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

  • Bing Li

    (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China)

Abstract

Response surface methodology was employed to optimise the ultrasonic-assisted extraction of protein from brewer's spent grain. Three variables, namely the extraction time (min), ultrasonic power (W/100 ml of extractant), and solid-liquid ratio (g/100 ml) were investigated. Optimal conditions were determined and tri-dimensional response surfaces were plotted using mathematical models. The ANOVA analysis indicated that all the quantities determined, i.e. the extraction time, ultrasonic power, and solid-liquid ratio, had significant positive linear and negative quadratic effects on the protein yield. Optimum conditions for the extraction of protein were found to be: the extraction time of 81.4 min, ultrasonic power of 88.2 W/100 ml of extractant, and solid-liquid ratio of 2.0 g/100 ml. The optimal predicted protein yield obtained was 104.2 mg/g BSG while the experimental yield of protein was in agreement with the predicted value.

Suggested Citation

  • De-Song Tang & Ying-Juan Tian & Yuan-Zhe He & Lin Li & Song-Qing Hu & Bing Li, 2010. "Optimisation of ultrasonic-assisted protein extraction from brewer's spent grain," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 28(1), pages 9-17.
    • Handle: RePEc:caa:jnlcjf:v:28:y:2010:i:1:id:178-2009-cjfs
    DOI: 10.17221/178/2009-CJFS
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