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Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

Author

Listed:
  • Igor Hochel

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

  • Jiří Škvor

    (Department of Medical Biophysics, 1 st Medical Faculty, Charles University in Prague, Prague, Czech Republic)

Abstract

An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.

Suggested Citation

  • Igor Hochel & Jiří Škvor, 2009. "Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 66-74.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:206-2009-cjfs
    DOI: 10.17221/206/2009-CJFS
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