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Methods for the Determination of Allergenic Substances in Foods

Author

Listed:
  • K. Tomková

    (SEDIUM RD, s.r.o., 530 02 Pardubice, Czech Republic)

  • F. Štumr

    (SEDIUM RD, s.r.o., 530 02 Pardubice, Czech Republic)

  • P. Dvorská

    (SEDIUM RD, s.r.o., 530 02 Pardubice, Czech Republic)

  • P. Šafářová

    (SEDIUM RD, s.r.o., 530 02 Pardubice, Czech Republic)

  • J. Rysová

    (Food Research Institute Prague, 102 31 Prague, Czech Republic)

  • D. Gabrovská
  • P. Hanák

    (Food Research Institute Prague, 102 31 Prague, Czech Republic)

  • J. Plicka

    (Immunotech a Beckman Coulter Company, 102 27 Prague, Czech Republic)

Abstract

Within the framework of the research project ELISA methods for the quantitative determination of allergenic substances in foodstuff and raw materials were developed. ELISA kits for allergenic proteins of milk (casein, beta-lactoglobulin and BSA) egg white proteins and mustard proteins were validated and collaborative studies were performed to prove the validation of the ELISA methods developed. Various methods of extraction were tested. The parameters as a limit of detection, as a limit of quantification, robustness, repeatability and accuracy were determined. A broad range of zero matrices for allergens were tested as well. The ELISA kits are suitable for the determination of allergens according to EU legislation Directive 2005/26/EC and Directive 2006/142/EC in the laboratories focused on this topic.

Suggested Citation

  • K. Tomková & F. Štumr & P. Dvorská & P. Šafářová & J. Rysová & D. Gabrovská & P. Hanák & J. Plicka, 2009. "Methods for the Determination of Allergenic Substances in Foods," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 369-371.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue1:id:945-cjfs
    DOI: 10.17221/945-CJFS
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