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Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation

Author

Listed:
  • M. Dušková

    (Department of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences Brno, 612 42 Brno, Czech Republic)

  • A. Španová

    (Institute of Experimental Biology, Masaryk University, 602 00 Brno, Czech Republic)

  • V. Dráb

    (Dairy Research Institute Ltd., 160 00 Praha, Czech Republic)

  • B. Rittich

Abstract

In this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as a DNA probe for the screening of homologous DNA sequences in 7 Lactococcus lactis subsp. lactis strains from the Culture Collection of Dairy Microorganisms (CCDM). Dot blot hybridisation and hybridisation of colonies were used for DNA/DNA hybridisation. It was shown that 6 tested strains of Lactococcus lactis subsp. lactis have genes encoding nisin. One strain had probably a defective gene encoding nisin.

Suggested Citation

  • M. Dušková & A. Španová & V. Dráb & B. Rittich, 2009. "Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(SpecialIs), pages 366-368.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue1:id:603-cjfs
    DOI: 10.17221/603-CJFS
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