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Influence of glycation and pepsin hydrolysis on immunoreactivity of albumin/globulin fraction of herbicide resistant wheat line

Author

Listed:
  • András Nagy

    (Department of Biology, Central Food Research Institute, Budapest, Hungary
    Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Katarzyna Marciniak-Darmochwał

    (Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Stanisław Krawczuk

    (Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland)

  • Dagmara Mierzejewska

    (Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Henryk Kostyra

    (Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Éva Gelencsér

    (Department of Biology, Central Food Research Institute, Budapest, Hungary)

Abstract

The aim of this study was to investigate the influence of non-enzymatic glycosylation on the immunogenic properties of soluble wheat proteins. Albumin/globulin fractions of herbicide resistant wheat line were non-enzymatically glycosylated using glucose for seven days at 37°C. The changes in their structures and immunoreactivity were then determined. The protein fractions were also hydrolysed with pepsin to determine the resistance to digestion. Albumin/globulin fractions before and after non-enzymatic glycosylation were analysed using o-phthaldialdehyde method and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The immunoreactivity of the protein fractions was determined using enzyme-linked immunosorbent assay methods, as well as affinity chromatography. The soluble wheat proteins showed smaller amounts of available α-amino groups after non-enzymatic glycosylation, and were stronger immunogens after glycation, but their antigenicity was not been affected significantly. However, pepsin hydrolysis of wheat proteins decreased their immunoreactivity.

Suggested Citation

  • András Nagy & Katarzyna Marciniak-Darmochwał & Stanisław Krawczuk & Dagmara Mierzejewska & Henryk Kostyra & Éva Gelencsér, 2009. "Influence of glycation and pepsin hydrolysis on immunoreactivity of albumin/globulin fraction of herbicide resistant wheat line," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(5), pages 320-329.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:5:id:48-2008-cjfs
    DOI: 10.17221/48/2008-CJFS
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