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The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs

Author

Listed:
  • Josef Brychta

    (State Veterinary Institute, Jihlava, Czech Republic)

  • Jiří Smola

    (Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Petr Pipek

    (Department of Food Preservation and Meat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

  • Jaroslav Ondráček

    (Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Vladimír Bednář

    (Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic)

  • Alois Čížek

    (Department of Food Preservation and Meat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

  • Tomáš Brychta

    (Department of Food Preservation and Meat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic)

Abstract

Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar were identified as B. cereus. The 85 isolates were examined for the enterotoxin production using both TECRA-VIA and BCET-RPLA kits (ELISA - 47, RPLA - 38). Thirty two isolates (66%) were positive for enterotoxin using the ELISA test while only 15 isolates (39%) gave positive results in the RPLA test system. In total, 178 (91.8%) and 164 (84%) of the strains isolated in our laboratory (from various foods) were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Parallel enterotoxin positive results obtained using both tests were demonstrated in only 9 isolates from 19 assessed (47.4%). Coincidental negative results from both kits were established for 3 isolates (15.8%) only. The isolates of B.cereus from meat were resistant to cephalothin (57%), clindamycin (14%), oxytetracycline (14%), and erythromycin (7%). The isolates from swabs were resistant to cephalothin (83%), erythromycin (16%), clindamycin (16%) and enrofloxacin (16%).

Suggested Citation

  • Josef Brychta & Jiří Smola & Petr Pipek & Jaroslav Ondráček & Vladimír Bednář & Alois Čížek & Tomáš Brychta, 2009. "The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(4), pages 284-292.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:4:id:84-2009-cjfs
    DOI: 10.17221/84/2009-CJFS
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