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Separation of low molecular weight rapeseed proteins by RP-HPLC-DAD - a short report

Author

Listed:
  • Agnieszka Kosińska

    (Department of Food Analysis, Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland)

  • Uttam D. Chavan

    (Biotechnology Centre, Mahatma Phule Agricultural University, Rahuri, India)

  • Ryszard Amarowicz

    (Department of Food Analysis, Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland)

Abstract

Low molecular weight proteins were extracted and isolated from rapeseed and analysed using the HPLC-DAD method. The separation of proteins and phenolic compounds was done on the reversed phase C18 column with a gradient of acetonitrile in water. The chromatogram was characterised by two peaks of low molecular weight proteins with the retention times of 19.92 and 23.24 min. Additional three main peaks of phenolic constituents were recorded on the chromatogram. One of them with maximum of UV spectrum at 328 nm was identified as sinapic acid derivatives.

Suggested Citation

  • Agnieszka Kosińska & Uttam D. Chavan & Ryszard Amarowicz, 2006. "Separation of low molecular weight rapeseed proteins by RP-HPLC-DAD - a short report," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 24(1), pages 41-44.
    • Handle: RePEc:caa:jnlcjf:v:24:y:2006:i:1:id:3292-cjfs
    DOI: 10.17221/3292-CJFS
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    Citations

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    Cited by:

    1. Ryszard Amarowicz & Ronald B. Pegg & Agnieszka Kosińska, 2009. "SE-HPLC separation of myosin complex with tannins from bearberries (Arctostaphylos uva-ursi L. Sprengel) leaves - a short report," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(5), pages 386-391.
    2. Magdalena Karamać & Eva Sendrejová & Agnieszka Kosińska & Dana Urminská, 2007. "Presence of ferulic acid in wheat glutenin fraction and its enzymatic hydrolysates - a short report," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 25(6), pages 359-364.

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