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Application of ionising radiation for the stabilisation of Trichoderma viride cellulases

Author

Listed:
  • Vít Plaček

    (Department of Radiation Applications, Nuclear Research Institute, Řež, Czech Republic)

  • Karel Vacek

    (Department of Radiation Applications, Nuclear Research Institute, Řež, Czech Republic)

  • Jan Káš

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

  • Kateřina Demnerová

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

  • Jarmila Zídková

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

  • Jiří Sajdok

    (Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic)

Abstract

The solutions of cellulolytic enzymes designated as standards for the cellulase activity assay were exposed in sealed glass ampoules (containing at least 100 Cx-units per ml in 30% w/w glycerol) to gamma radiation within the dose interval of 0-18 kGy. Glycerol was found to be the best enzyme stabiliser, however, the dose for the decontamination had to be increased in comparison with the original solution because glycerol protected also the contaminating microflora. The preparation after such treatment (30% of glycerol, dose 7 kGy) retained about 95% of the initial enzymatic activity without any decrease taking place in the following 6 months. The loss of the side activities did not exceed 10.5% and no bacterial contamination was detected either after 6 months of storage following the irradiation. No difference was found in the immunoreactivity of cellulases or in protein chromatografic (FPLC) pattern between the original and the irradiated enzyme preparations.

Suggested Citation

  • Vít Plaček & Karel Vacek & Jan Káš & Kateřina Demnerová & Jarmila Zídková & Jiří Sajdok, 2005. "Application of ionising radiation for the stabilisation of Trichoderma viride cellulases," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 23(3), pages 111-115.
    • Handle: RePEc:caa:jnlcjf:v:23:y:2005:i:3:id:3379-cjfs
    DOI: 10.17221/3379-CJFS
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