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Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

Author

Listed:
  • M. Vítková

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • Z. Macková

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • R. Koblovská

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

  • O. Lapčík

    (Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic)

Abstract

The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I50 values of the standard curves range between 0.3-1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid molecules is negligible. The immunoassays have been used for monitoring of changes in isoflavone levels in alimentary important plants, such as Medicago sativa, during germination; and during different vegetation stages of the Rutaceae family plants.

Suggested Citation

  • M. Vítková & Z. Macková & R. Koblovská & O. Lapčík, 2004. "Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 22(SpecialIs), pages 199-202.
    • Handle: RePEc:caa:jnlcjf:v:22:y:2004:i:specialissue:id:10659-cjfs
    DOI: 10.17221/10659-CJFS
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