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The determination of ferulic acid in sugar beet pulp

Author

Listed:
  • P. Jankovská

    (Institute of Chemical Technology - Department of Carbohydrates Chemistry and Technology, Prague, Czech Republic)

  • J. Čopíková

    (Institute of Chemical Technology - Department of Carbohydrates Chemistry and Technology, Prague, Czech Republic)

  • A. Sinitsya

    (Institute of Chemical Technology - Department of Carbohydrates Chemistry and Technology, Prague, Czech Republic)

Abstract

The content of ferulic acid in sugar beet pulp was determined by reverse phase high-pressure liquid chromatography (HPLC) and UV/VIS-spectroscopy. The acid extracts of pectin carrying feruloyl groups were prepared for analysis. To release ferulic acid from pectin the hydrolysis in alkaline medium (pH = 12.5) was performed. Both non-hydrolysed and hydrolysed extracts were measured by UV/VIS-spectroscopy after pH adjustment to the value of 10. The absorbance maximum was observed at 372 nm (ester of ferulic acid) for non-hydrolysed extracts and at 345 nm (sodium ferulate) for hydrolysed extracts. The HPLC estimation of ferulic acid was made in hydrolysed extracts only. The content of ferulic acid in sugar beet pulp was in the range of 0.3-0.9% (m/m). The data obtained by application of the particular methods to one set of samples were statistically compared. The results of all methods were in good agreement with each other\

Suggested Citation

  • P. Jankovská & J. Čopíková & A. Sinitsya, 2001. "The determination of ferulic acid in sugar beet pulp," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 19(4), pages 143-147.
    • Handle: RePEc:caa:jnlcjf:v:19:y:2001:i:4:id:6598-cjfs
    DOI: 10.17221/6598-CJFS
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