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The determination of isoflavones and coumestrol by capillary electrophoresis

Author

Listed:
  • J. Moravcová

    (Institute of Chemical Technology - Department of Chemistry of Natural Compounds, Prague, Czech Republic)

  • T. Kleinová

    (Institute of Chemical Technology - Department of Chemistry of Natural Compounds, Prague, Czech Republic)

Abstract

The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DAD detection. A baseline separation of all analytes except a pair, formononetin-biochanin A was achieved at pH 10.5 in 25 min. Detection limits were low (0.1 µg/ml) and the linearity of the detector response was established in the concentration range 0.4-60 µg/ml (180 µg/ml for coumestrol). Coumestrol was synthesized and the carbon signals in 13C-NMR spectrum of both coumestrol and di-O-acetylcoumestrol were assigned for the first time using two-dimensional HMQC technique.

Suggested Citation

  • J. Moravcová & T. Kleinová, 2001. "The determination of isoflavones and coumestrol by capillary electrophoresis," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 19(4), pages 132-138.
    • Handle: RePEc:caa:jnlcjf:v:19:y:2001:i:4:id:6596-cjfs
    DOI: 10.17221/6596-CJFS
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