IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v18y2000i2id8307-cjfs.html
   My bibliography  Save this article

Capillary electrophoretic and liquid chromatographic determination of synthetic dyes in non-alcoholic drinks and wine samples

Author

Listed:
  • I. Jančářová
  • A. Vondráčková
  • A. Šlampová
  • V. Kubáň

Abstract

Synthetic dyes in wine, juices, lemonades, instant and non-alcoholic drinks were determined by capillary electrophoresis (CE) and liquid chromatography (HPLC) with UV-VIS diode-array detection (DAD). The results were in the very good agreement (the relative deviations D < 16%, D < 14% and D < 15% between CE/UV-VIS, CE/HPLC and HPLC/UV-VIS, respectively, D < 6% in majority of samples) with those obtained by UV-VIS spectrophotometry. A borate/phosphate buffer of pH 9.0 (12.5mM borate and 12.5mM phosphate) containing 40mM sodium dodecylsulfate (SDS) was selected as an electrolyte for CE. A 50mM phosphate buffer and 5mM tetrabutylammonium hydroxide (TBAOH) water solution of pH 4.2 in 38% (v/v) acetonitrile were used as a mobile phase for isocratic HPLC separation on Sepharon SGX C18 (3 × 150 mm, 5 µm) column. RSDs for individual colorants were < 0.5% for migration times, < 3% for electrophoretic peak areas, < 0.9% for retention times and < 2.4% for HPLC peak areas. Limits of quantitations (LOQs for 10 S/N) were 1.5-3.3 mg/l for CE when using 50 µm I.D. separation capillary or 0.2 to 0.4 mg/l for HPLC.

Suggested Citation

  • I. Jančářová & A. Vondráčková & A. Šlampová & V. Kubáň, 2000. "Capillary electrophoretic and liquid chromatographic determination of synthetic dyes in non-alcoholic drinks and wine samples," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 18(2), pages 41-48.
    • Handle: RePEc:caa:jnlcjf:v:18:y:2000:i:2:id:8307-cjfs
    DOI: 10.17221/8307-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/8307-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/8307-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/8307-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:18:y:2000:i:2:id:8307-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.