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Molecular Detection of Beta Lactamase Genes from Salmonella Typhimurium Isolated from Poultry Droppings in Nyanya, Abuja

Author

Listed:
  • Kanu N.V.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Orji S.L.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Okafor H.A.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Adama R. I.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Mustapha, F.A.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Oluwafemi M. P.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Offiong A. B.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Tsebam B.C.

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Donli, S.C

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

  • Ekwe E.O

    (National Biotechnology and Research Development Agency, Lugbe Abuja, Nigeria)

Abstract

Salmonella infections are one of the major global public health problems. During the last decade, antibiotic resistance and multiresistance of S. typhimurium. This study aimed at molecular detection of Beta Lactamase genes from S. typhimurium Isolated from poultry droppings in Nyanya, Abuja. Fecal droppings samples were collected from two different Poultry farms using sterile screw tubes. S. Typhimurium was isolated and identified using standard microbiology methods. Antimicrobial susceptibility was carried out using Clinical and Laboratory Standards Institute method. Out of 180 samples collected the overall occurrence of S. typhimurium was 20 (11.1%). S. typhimurium isolated were higher in Farm A 9(10.0%) than in farm B 11(12.2%). The S. typhimurium isolates from Farm A were highly resistant to Ampicillin with 77.7% but less resistant to gentamicin with 33.3%, ofloxacin, ciprofloxacin and Sulphamethoxazole/trimethoprim with 44.4%. Isolates from Farm B were highly resistant to Ampicillin and Sulphamethoxazole/trimethoprim 81.8%, but less resistant to Cefuroxime 36.3%. the most common phenotypic resistant observed were C-CIP-CAZ-CRO-AUG- OFL -SXT- CEF (22.2%) from Farm A. CIP-CRO-CAZ- AMP -AUG- OFL -SXT- CEF (18.1%) from Farm B, The commonest MAR index observed in this study was 0.8(33.3%) in farm A and (36.3%) in farm B. The order of occurrences of the 4 Beta Lactamase genes detected was: blaCTX-M-9 and blaSHV (75.0%) > bla CMY2 and Bla CTX-M (50.0%). The resistance observed in this study indicates it will be difficult to use commonly use antibiotics to treat infection caused by S. typhimurium isolated in the study location

Suggested Citation

  • Kanu N.V. & Orji S.L. & Okafor H.A. & Adama R. I. & Mustapha, F.A. & Oluwafemi M. P. & Offiong A. B. & Tsebam B.C. & Donli, S.C & Ekwe E.O, 2024. "Molecular Detection of Beta Lactamase Genes from Salmonella Typhimurium Isolated from Poultry Droppings in Nyanya, Abuja," International Journal of Research and Innovation in Applied Science, International Journal of Research and Innovation in Applied Science (IJRIAS), vol. 9(8), pages 211-222, August.
    • Handle: RePEc:bjf:journl:v:9:y:2024:i:8:p:211-222
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