Thin Cell Layer Culture of Phalaenopsis sp. and Rhynchostylis sp

Phalaenopsis sp: The corm of shoot tip from elite shoot 90-day-old was used as material on the medium of MS + BA (1mg/l) + NAA (0.1mg/l) to rise to 2-4 PLB/corm after 90 days cultivation. PLBs were used as materials for in vitro propagation. PLB was cut into thin slices and cultures on the PLB initiation medium MS + peptone (1g/l) + BA (1mg/l) + NAA (0.1mg/l). After 3 months, PLB was initiated on different samples with 11.8 PLB/corm shoot tip sample, 7.4 PLB/leaf sample, and 6.2 PLB/thin slice of PLB. PLB has multiplied on the medium MS + BA (1mg/l) + NAA (0.1mg/l) to rise 11,4 PLB/sample. PLB was regenerated and rooted on the medium MS + NAA (0.1mg/l) with a high rate of 100%. The thin cell layer culture of Phalaenopsis sp. was established. Rhynchostylis sp: Shoot in 60-day-old clone in the pot was used as material for set up in vitro shoots by cultured on the medium MS + 2iP (1mg/l) + IBA (0,5mg/l) to raise 4-6 shoot/sample after 30 days of cultivation. Young shoots were multiplied on the medium MS + BA (1mg/l) + IBA (0.1mg/l) to produce 5-8 shoots/sample. The young shoot released young leaves and corms of shoot tip meristem used as material in thin cell layer culture. The corm shoot tip meristem was cultured on medium MS + BA (1mg/l) + IBA (0,5mg/l) to rise 4.8 shoots/sample; and young leaves were cultured on medium MS + 2iP (1mg/l) + IBA (0,5mg/l) to rise 2.4 PLB/sample and 3.6 shoots/sample. Shoots were multiplied on medium MS + BA (1mg/l) + IBA (0,5mg/l) and PLB was regenerated and rooted on medium MS + IBA (0,5mg/l). The thin cell layer culture of Rhynchostylis sp. was established.