Motility, Viability and Fertility of Indonesian Native Rooster Spermatozoa in Skim-Milk Based Semen Extender

The purpose of this study was to determine the motility and fertility of kampung rooster spermatozoa diluted in skim-milk based semen diluent. Semen that was collected from 12 kampung roosters, collected in one tube, then divided into six treatment groups, namely P0 = semen + skim milk extender; P1= semen + skimmed milk extender + 50 mM Glucose; P2= semen + skimmed milk extender + Ringer’s Lactate (4:1); P3= semen + skimmed milk extender + Ringer Lactate + 50 mM Glucose; P4= semen + skim milk extender + Physiological saline (4:1), and P5= semen + skim milk extender + Physiological saline (4:1) + Glucose 50 mM. All treated semen samples were stored at a refrigeration temperature of 3–5°C for 1 hour. Motility and viability were then observed under a microscope. This process was repeated four times. Subsequently, a single insemination was performed in the afternoon for each treatment group, with 10 Isa Brown hens aged 50 weeks assigned per group (n=60). This study used a completely randomized design (CRD). The results showed that the average of motility (%) of each treatment was: 81.25± 2.50; 83.75±4.79; 85.00±4.10; 86.25±2.50; 86.25±2.50 and 87.50±2.89. Average of viability was: 86.25±2.63; 88.75±4.11; 90.00±3.70; 90.25±6.80; 91.00±2.70 and 92.25±2.22. Average of fertility (%) was: 83.33±6.60; 85.10±8.32; 85.41±8.32; 92.85±6.58; 84.78±6.83 and 86.67±6.78. It can be concluded that the six extenders under study did not have significant effects on motility, viability, or fertility, indicating that all six skim-milk based extenders are suitable for use as extenders for fresh Kampung rooster semen.