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Identification of Vibrio Cholerae using Analytical Profile Index (API 20E)

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  • Christopher E. Ezeamagu

    (Chemistry Department, Federal College of Education, Technical Gusau, Zamfara State.)

  • Mande Garuba

    (Chemistry Department, Federal College of Education, Technical Gusau, Zamfara State.)

  • Toyosi F. Osisami

    (Department of Microbiology, Babcock University, Nigeria.)

Abstract

Cholera outbreak have resulted in the deaths of millions of people globally. The cholera-causing Vibrio cholerae, has multiple serogroups, and produces epidemics when two strains, O1 and O139, are present. With the exception of recent, rare instances, V. cholerae O139 caused prior outbreaks; however, V. cholerae O1 was responsible for all following epidemics. Hence, identification of the V. cholerae is an important step in the control of cholera outbreak especially in the local community where inadequate facilities for prognosis limit prompt treatment of patients. A total of 15 isolates previously identified with molecular method were subjected to TCBS Agar and Analytical Profile Index. All the sucrose-fermenting colonies on TCBS agar were correctly identified with API kit. The study concluded that API kit can be substituted for molecular method in prognosis of Cholera outbreak especially in resource-limited Countries due cost implication.

Suggested Citation

  • Christopher E. Ezeamagu & Mande Garuba & Toyosi F. Osisami, 2024. "Identification of Vibrio Cholerae using Analytical Profile Index (API 20E)," International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 9(5), pages 108-111, May.
    • Handle: RePEc:bjc:journl:v:9:y:2024:i:5:p:108-111
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