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Industrial Propagation of Phalaenopsis sp. by Bioreactor Technique

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  • Tran Van Minh

    (Production, Analytical and Laboratory Management, Federal Institute of Industrial Research, Oshodi, F.I.I.R.O., Lagos-Nigeria)

Abstract

Protocorm like bodies (PLB) were used as planting materials. Somatic embryo callus were initiated on medium supplemented with NAA (1mg/l) or 2.4D (1mg/l). Somatic cell suspension were cultured for initiation and for proliferation. on medium MS supplemented with NAA (1mg/l) and NAA (0.5mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS supplemented with NAA (0.5mg/l). Embryogenic suspension was stimulated on the medium MS supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of phalaenopsis were regeneration on the medium MS supplemented with BA (0.5mg/l) + NAA (0.1mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26+2oC, 11,1-22,2μmol/m2/s, and 30rpm. Micropropagation of phalaenopsis sp. by bioreactor technique was set up to produce 7,580 plantlets per one liter of somatic embryogenesis.

Suggested Citation

  • Tran Van Minh, 2023. "Industrial Propagation of Phalaenopsis sp. by Bioreactor Technique," International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 8(4), pages 29-39, April.
    • Handle: RePEc:bjc:journl:v:8:y:2023:i:4:p:29-39
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