The Beneficial Effect of The Yeast Enriched with Nano Zinc Particles on Mineral Status of Grape Seedlings

This investigation included two integrated experiments, the first one is the microbiological experiment that carried out in Microbial Biotechnology Department, NRC, Cairo, Egypt, where multiple incorporation strategies were used for production zinc oxide Nanoparticles by enrichment of yeast with zinc including; (1): In the first procedure, zinc was added to the liquid medium just after the yeast was inoculated (growth phase), (2): For integration, it was added after 24 h of incubation (non-growth phase) in the second procedure. The obtained results showed that the first method (growth phase) was adopted in order to reduce the chance of medium contamination. Zinc sulphate has no passive effect on yeast cell biomass. The second experiment is horticultural one which was done in National Research Centre greenhouse on Flame seedless grape seedlings which included five treatments as follows: control (spraying with water only), frozen yeast enriched with zinc (as foliar spray), active yeast enriched with zinc (as foliar spray), frozen yeast enriched with zinc (as soil application) and active yeast enriched with zinc (as soil application). When zinc content in the grape leaves as ppm was examine after 15 days, the results showed that active fresh yeast enriched with zinc that treated as foliar spray recorded the highest rate of zinc in grape leaves (180 ppm), followed by active fresh yeast enriched with zinc as soil application which recorded 170.8 ppm. On the other hand, the untreated plants (control) showed the lowest value of zinc in the leaves (91.6 ppm) when compared with the other treatments.