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Exploring the Changes of Resistant Genes Expression in Groundnuts Arachis Hypogea In Response to Aspergillus Flavus Exposure at Seedling Stage

Author

Listed:
  • Robert O. Okayo

    (Department of Plant, Animal and Food Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, Kenya)

  • Darius O. Andika

    (Department of Plant, Animal and Food Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, Kenya)

  • Mathews M. Dida

    (Department of Applied Sciences, Maseno University, Maseno, Kenya)

  • George O. K’Otuto

    (Department of Applied Sciences, Maseno University, Maseno, Kenya)

  • Bernard M. Gichimu

    (Department of Agricultural Resource Management, University of Embu, Embu, Kenya)

Abstract

Aspergillus flavus infect groundnut seeds and produce secondary metabolites, aflatoxins. The aflatoxins are associated with various diseases in domestic animals and humans globally. Mitigating the aflatoxin contamination in crops through the development of cultivars tolerant to fungus colonization and aflatoxin contamination has been considered the most cost-effective measure. This research was conducted to ascertain that the resistance genes identified in the previous transcriptome analysis were involved in groundnut defense mechanisms to A. flavus infection. Eight genes were selected for additional scrutiny through the real time PCR on a groundnut seedling at an interval of 2 days within a 7-day period. The results indicate a network of gene expression patterns in a sequential order in both resistance and susceptible lines at a seedling stage. The peak expression level per gene indicates the time gene action was crucial. We conclude that these genes are involved in groundnut resistance to A. flavus infection and provide important targets for the molecular marker screening.

Suggested Citation

  • Robert O. Okayo & Darius O. Andika & Mathews M. Dida & George O. K’Otuto & Bernard M. Gichimu, 2020. "Exploring the Changes of Resistant Genes Expression in Groundnuts Arachis Hypogea In Response to Aspergillus Flavus Exposure at Seedling Stage," International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 7(7), pages 203-208, July.
    • Handle: RePEc:bjc:journl:v:7:y:2020:i:7:p:203-208
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