Rapid Detection of Tuberculosis using Urine Samples: Development & Validation of ELISA Technique

Diagnosis of tuberculosis (TB) and its early detection can be possible only when an individual permits itself for early diagnosis. In the recent study, the serum elevated against Mycobacterium tuberculosis antigen was settled for the diagnosis of tuberculosis infection employing serological tests. The mycobacterial proteins was identified based on indirect ELISA including high sensitivity of 0.1 mg and the protein ranging from 20 kDa to 150 kDa exhibit immunoreactivity in western blotting applying TB serum. Correlation coefficient among various phases of infected urine samples assemble from different regions of Odisha with the titres of Ab was seized as the primary end point for the regularity of protocol. (Maximal correlation r = 0.999) was detected in phase III infected urine sample assemble from Mayurbhanj, whereas minimal low correlation along with TB serum (r = 0.703) was marked in phase I infected urine sample assemble from Balasore. (Linear positive correlations R2) present within antigenic proteins at all the phases with TB serum. Henceforth, the TB serum could be used for the early detection of TB. This immuno serological accesses us to design a robust, highly sensitive & specific with accuracy diagnostic kit for initial detection of tuberculosis infection.