Author
Listed:
- Attah Ambrose Abuh
(Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)
- Ishaleku David
(Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)
- Nkene Istifanus Haruna
(Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)
- Igbawua Isaac Nyiayem
(Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)
- Datok, Danladi Walong
(Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)
Abstract
Fluoroquinolones have been the drug of choice for the treatment of Salmonellla infections in humans and animals. The emergence of fluoroquinolone resistant Salmonella strains has posed significant treatment challenges worldwide. This research is aimed at detecting the Plasmid mediated quinolone resistant genes of Salmonella typhi isolated from stool samples of patients attending selected Primary Health Centres in Kaduna metropolis. Methodology: A total of 200 stool samples were randomly collected from patients with suspected Typhoid fever using standard protocols. Salmonella typhi was isolated from the samples using standard culture and microbiological techniques. Antibiotics susceptibility testing was done using the disc diffusion method to investigate the ability of Salmonella typhi to resist the tested antibiotics. The bacterial genome was extracted and resistant genes were detected using PCR. Results. Out of the 200 stool samples, 61(30.5%) Salmonella typhi was isolated. Out of the 61 isolates, 44 (72.1%) carried resistant genes of which 6 of the isolates carried the Ciprofloxacin resistance genes. The genes that were discovered mainly were QnrB, aac(6’)-Ib-cr-R and QepA. Conclusion: The antibiotic susceptibility testing of Salmonella typhi in this study showed an increasing trend of plasmid mediated quinolone resistance (PMQR). The allele B of qnr gene was found to be the predominant cause of PMQR in this study.
Suggested Citation
Attah Ambrose Abuh & Ishaleku David & Nkene Istifanus Haruna & Igbawua Isaac Nyiayem & Datok, Danladi Walong, 2024.
"Molecular Detection of Plasmid-Mediated Quinolone Resistance in Salmonella Typhi Isolated from Stool of Patients Attending Selected Primary Health Centres in Kaduna Metropoilis, Nigeria,"
International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 11(9), pages 1290-1300, September.
Handle:
RePEc:bjc:journl:v:11:y:2024:i:9:p:1290-1300
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:bjc:journl:v:11:y:2024:i:9:p:1290-1300. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Dr. Renu Malsaria (email available below). General contact details of provider: https://rsisinternational.org/journals/ijrsi/ .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.