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In vitro Regeneration protocol for Mulberry Morus alba L. through Tissue Culture Techniques

Author

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  • Md. Sohel Rana

    (Regional Bamboo Research and Training Centre, Bangladesh Forest Research Institute, Domar, Nilphamari-5340, Bangladesh)

  • Muhammad Nurul Amin

    (Professor, Department of Botany, University of Rajshahi-6205, Bangladesh)

  • Mustafa Abul Kalam Azad

    (Professor, Department of Botany, University of Rajshahi-6205, Bangladesh)

Abstract

This research work was taken for enormous production of white mulberry (Morus alba L.) through micropropagation techniques. Surface sterilization of the explants collected from the field grown mature plants was important and it was done with 0.1% HgCl₂ (Mercuric chloride) solution at different durations. Result showed that duration of the treatment was the best at 12 minutes for both shoot tips and nodal segments. Axillary shoot proliferation of nodal explants was better than that of (terminal) shoot tips. Data analysis showed that cytokinin BAP (6-Benzyl aminopurine) was more effective than Kn (Kinetin) for axillary shoot proliferation. The MS medium supplemented with 6.0 µM was the best to grow 100% shoot proliferation of the explants where the average length was 5.57cm ± 0.05. On the other hand, different concentration and type of auxin affected greatly to induce number and percentage of roots per shoot. In this case, half MS medium supplemented with 6.0 µM IBA (Indole-3-butyric acid) was showed the highest percentage (100%) of root regeneration where the average length was 4.21cm ± 0.07.

Suggested Citation

  • Md. Sohel Rana & Muhammad Nurul Amin & Mustafa Abul Kalam Azad, 2022. "In vitro Regeneration protocol for Mulberry Morus alba L. through Tissue Culture Techniques," International Journal of Research and Innovation in Social Science, International Journal of Research and Innovation in Social Science (IJRISS), vol. 6(1), pages 124-128, January.
    • Handle: RePEc:bcp:journl:v:6:y:2022:i:1:p:124-128
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