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Chromosomal analysis and Nors polymorphism of bagarius suchus (Siluriformes: Sisoridae) by conventional banding and fish techniques

Author

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  • Nuntiya Maneechot

    (University Muang KhonKaen, Thailand)

  • Weerayuth Supiwong

    (University Muang KhonKaen, Thailand)

  • Alongklod Tanomtong

    (University Muang KhonKaen, Thailand)

Abstract

In the present study, conventional staining and NORs banding as well as Fluorescence In Situ Hybridization (FISH) using the 18S rDNA and telomeric (TTAGGG)n probes were applied to stain the chromosomes of crocodile catfish, Bagariussuchus (Siluriformes, Sisoridae) from the Chao Phraya River, Thailand. Kidney cells of six male and six female crocodile catfishes were used as a sample. The mitotic chromosome preparations were done directly from kidney cells. The results showed that the diploid chromosome number of B. suchus was 2n=56, the Fundamental Numbers (NF) were 102 in both male and female. The karyotype comprises 17m+17sm+12a+10t. The Nucleolar Organizer Regions (NORs) were detected by Ag-NORs banding and 18S rDNA probe mapping. The 18S rDNA are terminally located on the short arm adjacent to the telomere of the single pair of the 1st chromosome pair whereas NOR-bearing chromosome is only one chromosome of the 1st chromosome pair (1a 1b, polymorphic characteristic) at the subtelomeric region of the short arm. Moreover, FISH with telomeric probe showed hybridization signals on each telomere of all chromosomes and interstitial telomeric sites were not detected. There were variations in signals of FISH and their position in the karyotype along with variation in DNA sequences. These markers are useful for future discrimination of population of closely related species and their polymorphism

Suggested Citation

  • Nuntiya Maneechot & Weerayuth Supiwong & Alongklod Tanomtong, 2016. "Chromosomal analysis and Nors polymorphism of bagarius suchus (Siluriformes: Sisoridae) by conventional banding and fish techniques," Journal of Applied and Physical Sciences, Prof. Vakhrushev Alexander, vol. 2(1), pages 10-19.
    • Handle: RePEc:apb:japsss:2016:p:10-19
    DOI: 10.20474/japs-2.1.2
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    Cited by:

    1. Arzu Ucar & Muhammed Atamanalp & Esat Mahmut Kocaman & Gonca Alak & Ahmet Topal & Ozden Fakioglu & Veysel Parlak & Telat Yanik, 2019. "Effects of dietary bentonite on improvements in hematology and enzyme in copper exposed rainbow trout (Oncorhynchus mykiss)," Journal of Applied and Physical Sciences, Prof. Vakhrushev Alexander, vol. 5(2), pages 67-72.
    2. Astri Nur Istyami & Made Tri Ari Penia Kresnowati & Tirto Prakoso & Tatang Hernas Soerawidjaja, 2018. "The use of hydrophobic beads in triglyceride hydrolysis," Journal of Advances in Technology and Engineering Research, A/Professor Akbar A. Khatibi, vol. 4(1), pages 09-16.
    3. Ahmadi, 2017. "The feasibility study on trapping experiments with lights in Barito river of Indonesia," Journal of Advances in Technology and Engineering Research, A/Professor Akbar A. Khatibi, vol. 3(6), pages 244-253.

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