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Study of Enzyme Purification Method and Growth Pattern for Pseudomonas putida in Mercury Removal

Author

Listed:
  • Abdul Aziz Mohd Azoddein

    (Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Pahang, Malaysia)

  • Siti Nur Baizura Yusuf

    (Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Pahang, Malaysia)

  • Faten Ahada Mohd Azli

    (Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Pahang, Malaysia)

  • Mariah Che Mamat

    (Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Pahang, Malaysia)

  • Mohamad Tahfiz Mohamad Tajuddin

    (Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Pahang, Malaysia)

Abstract

Mercury is one of the toxic element and very harmful. The exposure of the mercury will mainly cause health effect, and the exposure can be in term of dose, the age of person exposed, duration exposed route exposed and duration of exposure. In Malaysia, Mercury is one of the heavy metals of concern, found in wastewaters coming from oil refinery and petrochemical industries. Mercury and mercurial compounds are highly toxic contaminants in the aquatic systems and soils. The proper disposal of wastewater should be implemented with the Department of Environment of Malaysia (DOE) which is the concentration of mercury should be 0.05 ppm for standard B in wastewater for the sake of people and environmental health. There are many types of mercury removal technology to reduce the concentration of mercury such as chemical treatment, ion exchange, membrane filtration, adsorption as well as bioremediation which used for mercury removal in this study. This paper present the activity of enzyme from Pseudomonas putida (P. putida) is produced in the mercury treatment from petrochemical wastewater at optimum condition. P. putida growth at the optimum condition in the different mercury concentration was observed. As a conclusion of optimum condition concentration of mercury and shaker speed at 7 ppm is 41â—¦C and 180 rpm respectively, with ability to reduce 92.59% mercury concentration. During the processes of mercury removal, mercuric reductase was produced at a value of 56 kDa.

Suggested Citation

  • Abdul Aziz Mohd Azoddein & Siti Nur Baizura Yusuf & Faten Ahada Mohd Azli & Mariah Che Mamat & Mohamad Tahfiz Mohamad Tajuddin, 2018. "Study of Enzyme Purification Method and Growth Pattern for Pseudomonas putida in Mercury Removal," International Journal of Applied and Physical Sciences, Dr K.Vivehananthan, vol. 4(2), pages 69-75.
    • Handle: RePEc:apa:ijapss:2018:p:69-75
    DOI: 10.20469/ijaps.4.50005-2.pdf
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    References listed on IDEAS

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    1. Patama Bunruk & Duangporn Kantachote & Ampaitip Sukhoom, 2017. "Isolation and selection of purple non-sulfur bacteria for phosphate removal in rearing water from shrimp cultivation," Journal of Applied and Physical Sciences, Prof. Vakhrushev Alexander, vol. 3(2), pages 73-80.
    2. Peiravi Roya & Rahmatiyar Hadi & Alidadi Hossein & Mohammad Vahedian, 2015. "Survey of Wastewater Stabilization Pond Potential in Meeting Environmental Standards," International Journal of Applied and Physical Sciences, Dr K.Vivehananthan, vol. 1(1), pages 19-21.
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